World Library  

Add to Book Shelf
Flag as Inappropriate
Email this Book

Plos Genetics : Impact of Nonsense-mediated Mrna Decay on the Global Expression Profile of Budding Yeast, Volume 2

By Kruglyak, Leonid

Click here to view

Book Id: WPLBN0003923379
Format Type: PDF eBook :
File Size:
Reproduction Date: 2015

Title: Plos Genetics : Impact of Nonsense-mediated Mrna Decay on the Global Expression Profile of Budding Yeast, Volume 2  
Author: Kruglyak, Leonid
Volume: Volume 2
Language: English
Subject: Journals, Science, Genetics
Collections: Periodicals: Journal and Magazine Collection (Contemporary), PLoS Genetics
Publication Date:
Publisher: Plos


APA MLA Chicago

Kruglyak, L. (n.d.). Plos Genetics : Impact of Nonsense-mediated Mrna Decay on the Global Expression Profile of Budding Yeast, Volume 2. Retrieved from

Description : Nonsense-mediated mRNA decay (NMD) is a eukaryotic mechanism of RNA surveillance that selectively eliminates aberrant transcripts coding for potentially deleterious proteins. NMD also functions in the normal repertoire of gene expression. In Saccharomyces cerevisiae, hundreds of endogenous RNA Polymerase II transcripts achieve steady-state levels that depend on NMD. For some, the decay rate is directly influenced by NMD (direct targets). For others, abundance is NMD-sensitive but without any effect on the decay rate (indirect targets). To distinguish between direct and indirect targets, total RNA from wild-type (Nmdþ) and mutant (Nmd ) strains was probed with high-density arrays across a 1-h time window following transcription inhibition. Statistical models were developed to describe the kinetics of RNA decay. 45% 6 5% of RNAs targeted by NMD were predicted to be direct targets with altered decay rates in Nmd strains. Parallel experiments using conventional methods were conducted to empirically test predictions from the global experiment. The results show that the global assay reliably distinguished direct versus indirect targets. Different types of targets were investigated, including transcripts containing adjacent, disabled open reading frames, upstream open reading frames, and those prone to out-of-frame initiation of translation. Known targeting mechanisms fail to account for all of the direct targets of NMD, suggesting that additional targeting mechanisms remain to be elucidated. 30% of the protein-coding targets of NMD fell into two broadly defined functional themes : those affecting chromosome structure and behavior and those affecting cell surface dynamics. Overall, the results provide a preview for how expression profiles in multi-cellular eukaryotes might be impacted by NMD. Furthermore, the methods for analyzing decay rates on a global scale offer a blueprint for new ways to study mRNA decay pathways in any organism where cultured cell lines are available.


Click To View

Additional Books

  • Plos Genetics : a Kinome-wide Rnai Scree... (by )
  • Plos Genetics : Twist1 Suppresses Senesc... (by )
  • Plos Genetics : a Yeast Gsk-3 Kinase McK... (by )
  • Plos Genetics : Genome-wide Association ... (by )
  • Plos Genetics : a Densely Interconnected... (by )
  • Plos Genetics : Meta-analysis of Genome-... (by )
  • Plos Genetics : Consistent Association o... (by )
  • Plos Genetics : a Missense Mutation in a... (by )
  • Plos Genetics : Recombination Drives Ver... (by )
  • Plos Genetics : Evidence for Domesticate... (by )
  • Plos Genetics : the Stb Operon Balances ... (by )
  • Plos Genetics : Maps of Open Chromatin G... (by )
Scroll Left
Scroll Right


Copyright © World Library Foundation. All rights reserved. eBooks from National Public Library are sponsored by the World Library Foundation,
a 501c(4) Member's Support Non-Profit Organization, and is NOT affiliated with any governmental agency or department.