World Library  


Add to Book Shelf
Flag as Inappropriate
Email this Book

Plos Genetics : Maize Centromere Structure and Evolution ; Sequence Analysis of Centromeres 2 and 5 Reveals Dynamic Loci Shaped Primarily by Retrotransposons, Volume 5

By Malik, Harmit S.

Click here to view

Book Id: WPLBN0003927819
Format Type: PDF eBook :
File Size:
Reproduction Date: 2015

Title: Plos Genetics : Maize Centromere Structure and Evolution ; Sequence Analysis of Centromeres 2 and 5 Reveals Dynamic Loci Shaped Primarily by Retrotransposons, Volume 5  
Author: Malik, Harmit S.
Volume: Volume 5
Language: English
Subject: Journals, Science, Genetics
Collections: Periodicals: Journal and Magazine Collection (Contemporary), PLoS Genetics
Historic
Publication Date:
Publisher: Plos

Citation

APA MLA Chicago

Malik, H. S. (n.d.). Plos Genetics : Maize Centromere Structure and Evolution ; Sequence Analysis of Centromeres 2 and 5 Reveals Dynamic Loci Shaped Primarily by Retrotransposons, Volume 5. Retrieved from http://www.nationalpubliclibrary.com/


Description
Description : We describe a comprehensive and general approach for mapping centromeres and present a detailed characterization of two maize centromeres. Centromeres are difficult to map and analyze because they consist primarily of repetitive DNA sequences, which in maize are the tandem satellite repeat CentC and interspersed centromeric retrotransposons of maize (CRM). Centromeres are defined epigenetically by the centromeric histone H3 variant, CENH3. Using novel markers derived from centromere repeats, we have mapped all ten centromeres onto the physical and genetic maps of maize. We were able to completely traverse centromeres 2 and 5, confirm physical maps by fluorescence in situ hybridization (FISH), and delineate their functional regions by chromatin immunoprecipitation (ChIP) with anti-CENH3 antibody followed by pyrosequencing. These two centromeres differ substantially in size, apparent CENH3 density, and arrangement of centromeric repeats: and they are larger than the rice centromeres characterized to date. Furthermore, centromere 5 consists of two distinct CENH3 domains that are separated by several megabases. Succession of centromere repeat classes is evidenced by the fact that elements belonging to the recently active recombinant subgroups of CRM1 colonize the present day centromeres, while elements of the ancestral subgroups are also found in the flanking regions. Using abundant CRM and non-CRM retrotransposons that inserted in and near these two centromeres to create a historical record of centromere location, we show that maize centromeres are fluid genomic regions whose borders are heavily influenced by the interplay of retrotransposons and epigenetic marks. Furthermore, we propose that CRMs may be involved in removal of centromeric DNA (specifically CentC), invasion of centromeres by non-CRM retrotransposons, and local repositioning of the CENH3.

 

Click To View

Additional Books


  • Plos Genetics : Drosophila Tan Encodes a... (by )
  • Plos Genetics : Cohesin is Limiting for ... (by )
  • Plos Genetics : the Variant Rs1867277 in... (by )
  • Plos Genetics : Regulating Repression ; ... (by )
  • Plos Genetics : Non-coding Changes Cause... (by )
  • Plos Genetics : Terra Promotes Telomere ... (by )
  • Plos Genetics : Genome-wide Association ... (by )
  • Plos Genetics : the Rose-combmutation in... (by )
  • Plos Genetics : Designing Sirna That Dis... (by )
  • Plos Genetics : an Iterative Genetic and... (by )
  • Plos Genetics : a Feed-forward Loop Coup... (by )
  • Plos Genetics : Ancestry-shift Refinemen... (by )
Scroll Left
Scroll Right

 



Copyright © World Library Foundation. All rights reserved. eBooks from National Public Library are sponsored by the World Library Foundation,
a 501c(4) Member's Support Non-Profit Organization, and is NOT affiliated with any governmental agency or department.