World Library  


Add to Book Shelf
Flag as Inappropriate
Email this Book

Plos Genetics : Deep Sequencing of Plant and Animal Dna Contained Within Traditional Chinese Medicines Reveals Legality Issues and Health Safety Concerns, Volume 8

By Desalle, Robert

Click here to view

Book Id: WPLBN0003941684
Format Type: PDF eBook :
File Size:
Reproduction Date: 2015

Title: Plos Genetics : Deep Sequencing of Plant and Animal Dna Contained Within Traditional Chinese Medicines Reveals Legality Issues and Health Safety Concerns, Volume 8  
Author: Desalle, Robert
Volume: Volume 8
Language: English
Subject: Journals, Science, Genetics
Collections: Periodicals: Journal and Magazine Collection (Contemporary), PLoS Genetics
Historic
Publication Date:
Publisher: Plos

Citation

APA MLA Chicago

Desalle, R. (n.d.). Plos Genetics : Deep Sequencing of Plant and Animal Dna Contained Within Traditional Chinese Medicines Reveals Legality Issues and Health Safety Concerns, Volume 8. Retrieved from http://www.nationalpubliclibrary.com/


Description
Description : Traditional Chinese medicine (TCM) has been practiced for thousands of years, but only within the last few decades has its use become more widespread outside of Asia. Concerns continue to be raised about the efficacy, legality, and safety of many popular complementary alternative medicines, including TCMs. Ingredients of some TCMs are known to include derivatives of endangered, trade-restricted species of plants and animals, and therefore contravene the Convention on International Trade in Endangered Species (CITES) legislation. Chromatographic studies have detected the presence of heavy metals and plant toxins within some TCMs, and there are numerous cases of adverse reactions. It is in the interests of both biodiversity conservation and public safety that techniques are developed to screen medicinals like TCMs. Targeting both the p-loop region of the plastid trnL gene and the mitochondrial 16S ribosomal RNA gene, over 49,000 amplicon sequence reads were generated from 15 TCM samples presented in the form of powders, tablets, capsules, bile flakes, and herbal teas. Here we show that second-generation, high-throughput sequencing (HTS) of DNA represents an effective means to genetically audit organic ingredients within complex TCMs. Comparison of DNA sequence data to reference databases revealed the presence of 68 different plant families and included genera, such as Ephedra and Asarum, that are potentially toxic. Similarly, animal families were identified that include genera that are classified as vulnerable, endangered, or critically endangered, including Asiatic black bear (Ursus thibetanus) and Saiga antelope (Saiga tatarica). Bovidae, Cervidae, and Bufonidae DNA were also detected in many of the TCM samples and were rarely declared on the product packaging. This study demonstrates that deep sequencing via HTS is an efficient and cost-effective way to audit highly processed TCM products and will assist in monitoring their legality and safety especially when plant reference databases become better established.

 

Click To View

Additional Books


  • Plos Genetics : Discerning the Ancestry ... (by )
  • Plos Genetics : Expression Quantitative ... (by )
  • Plos Genetics : Full-length Synaptonemal... (by )
  • Plos Genetics : an Age-old Problem, Volu... (by )
  • Plos Genetics : Quantifying the Underest... (by )
  • Plos Genetics : Entropy Explains Aging, ... (by )
  • Plos Genetics : the Tale Class Homeobox ... (by )
  • Plos Genetics : Power to Detect Risk All... (by )
  • Plos Genetics : Ultra-deep Sequencing of... (by )
  • Plos Genetics : Inactivation of Chk2 and... (by )
  • Plos Genetics : Modulating the Strength ... (by )
  • Plos Genetics : Spastic Paraplegia Mutat... (by )
Scroll Left
Scroll Right

 



Copyright © World Library Foundation. All rights reserved. eBooks from National Public Library are sponsored by the World Library Foundation,
a 501c(4) Member's Support Non-Profit Organization, and is NOT affiliated with any governmental agency or department.